The Laboratory of Genomics and Immunobiology – LGBIO of the University of São Paulo in Ribeirao Preto city, São Pàulo State, Brazil has one opening for a postdoctoral fellow with funding of São Paulo Research Foundation – FAPESP, requiring experience with molecular biology techniques, recombinant protein production, vaccinology, immunology and parasitology. Candidates must be proactive, be fluent in English and have facility to work in team. The aim of the project is to develop an anti-tick vaccine for bovines and dogs. The group already has several patented antigens.
Activities of the awardee
1. Enlarge the colony of Rhipicephalus sanguineus ticks and Rhipicephalus microplus with infestations in rabbits and bovines and maintenance of unfed ticks;
2. Optimization and large scale production of recombinant proteins in E. coli, P. pastoris and new expression systems;
3. Preparation of immunogens and inoculation of animals (cattle, dogs and chickens) with recombinant proteins to evaluate vaccine efficacy and production of hyperimmune sera; 4. Perform artificial infestation of animals with ticks to evaluate the protection generated by the inoculation of recombinant proteins;
5. Evaluate the biological parameters and reproductive of ticks during the infestation in animals;
6. Collection and processing of blood, spleen and lymph nodes of tick-infested animals, as well as establishment of primary cell culture and in vitro stimulation lineages, aiming the analysis of humoral and cellular immunity stimulated by vaccination;
7. Perform experiments for determination of antigen-specific antibodies and cytokines by ELISA and flow cytometry;
8. Design and execution of activities mapping of B cell epitopes through peptide microarray, phage display and complementary techniques;
9. Implementation of new generation genetic sequencing techniques for characterization of bovine MHC alleles;
10. Design and execution of mapping activities of CD4 T cell epitopes through the characterization of bovine immuno-peptidomes by high resolution mass spectrometry;
11. Assist in the supervision of students and technicians, aid lab organization and the purchase of reagents and equipment’s;
12. Writing of reports and scientific articles.
The development of research activities will require frequent trips and stays in Viçosa, Minas Gerais State, for experiments of immunization in cattle, collection of samples, isolation, culture and freezing of cells.
The fellowship has duration of 12 up to 24 months; it is expected to start in 1st December, 2018. Mouse, rabbit, bovine and dog models will be utilized.
Note: Rules of the São Paulo Research Foundation-FAPESP for the Program of Fellowship linked to a Thematic Project can be found at http://www.fapesp.br/en/5427 and require exclusive dedication of 40 hours per week.
Interested candidates should send curriculum, three names to ask a recommendation letter, a letter explaining how his/her experience fits the project and a concise description of a research project under the fellowship to the Principal Investigator Prof. Beatriz Ferreira via email [email protected] . The deadline for applications is 4th of November. Final decision will be at 8th of November, 2018.
Desired skills and experience
The successful candidate will have a PhD in life sciences, preferably immunology or cell or molecular biology, with experience with molecular immunology and cell culture. Experience with handling animal models is desirable. The training environment is collaborative and includes both PhD and MD scientists, with participation in joint lab meetings and work in progress seminars. The candidate must have a strong track record of innovative and independent research in their previous training. Knowledge of at least basic Portuguese is desirable but not required.
About the employer
The Laboratory of Genomics and Immunobiology is located at the University of São Paulo at Ribeirão Preto College of Nursing, Ribeirão Preto, Brazil. We have a strong collaboration with the Department of Biochemistry and Immunology at Ribeirão Preto School of Medicine. Both basic and translational research is performed in a collaborative environment.
This opportunity is open to candidates of any nationalities. The selected candidate will receive a FAPESP’s Post-Doctoral fellowship in the amount of BRL 7.373,10 monthly and a research contingency fund, equivalent to 15% of the annual value of the fellowship which should be spent in items directly related to the research activity.
More information about the fellowship is at: http://www.fapesp.br/oportunidades/2428/
Post-doctoral Fellowship in Parasitology
The opportunity is for the project belonging to the Institutional Research Development Plan (PDIP), whose objective will be the development of scientific and technological solutions that will provide rapid and timely responses to strengthen laboratory actions in health surveillance. Project title: “Pre-clinical study of new candidate drugs against leishmaniasis”, coordinated by Dr. Andre Gustavo Tempone.
The present proposal aims to identify an approved drug by means of the drug repositioning or synthetic active compound based in a natural prototype, which presents promising anti-Leishmania infantum activity. The investigator will initially perform in vitro phenotypic assays using the intracellular amastigote model of Leishmania infantum to determine the activity of the compounds to be tested. Studies will be carried out in different cell lines to evaluate the mammalian cytotoxicity. Cellular studies, based on flow cytometry techniques, spectrofluorimetry and transmission electron microscopy will be used to investigate the possible mechanisms of action of the most promising compounds. Finally, in vitro pharmacokinetic studies (ADME) such as solubility, membrane permeability and metabolization will be performed as initial parameters for the design of animal model efficacy studies.
Pre-requisites for the position: Doctoral degree in area related to the project based on Drug Discovery in visceral leishmaniasis or Chagas disease; project experience involving techniques of phenotypic assays (intracellular amastigotes and cytotoxicity), experience in studies of cellular action mechanism; experience in pharmacokinetic profile studies and in vivo efficacy studies.
Candidates need to send an e-mail to the researcher Dr. Andre G. Tempone ([email protected] ), containing curriculum vitae, letters of presentation justifying the interest in the vacancy and letters of recommendation with contacts (name, institution, telephone and e-mail) of two researchers who can give information about the candidate.
The project will be funded by São Paulo Research Foundation – FAPESP (www.fapesp.br/en ). The vacancy is open to Brazilians and foreigners. The selected will receive FAPESP Post-Doctoral Fellowship (www.fapesp.br/en/postdoc ) in the amount of BRL7,373.10 per month and Technical Reserve. The Technical Reserve of PD Grant is equivalent to 15% of the annual value of the scholarship and is intended to meet unforeseen expenses directly related to the research activity.
More information about the fellowship is at: fapesp.br/oportunidades/2415 .
Post-doctoral Fellowship in Molecular Biology
The research group Bioproducts’ Production and Purification Lab (BioPPuL), located in the Department of Bioprocesses and Biotechnology of the FCFAr of UNESP, offers a postdoctoral fellowship to develop the recombinant microorganism for the production of L-asparaginase (ASNase) and studies of its production process by submerged cultivation.
About the project
Getting older is the biggest risk factor for most fatal diseases, including cancer, heart disease and Alzheimer’s. The advances in nanomedicine comprise the development of nanoparticles either for drug delivering or as platforms for biomolecules purification. Simultaneously, continuous progresses have been made for the development of recombinant therapeutic enzymes, namely L-asparaginase (LA), which presents antineoplastic properties for thetreatment of leukaemia’s, in particular for Acute Lymphoblastic Leukemia (ALL). Currently, the LA available in medical applications is produced from bacteria. However, this type of LA may activate the immunological system causing human hypersensitivity reactions. Therefore, the search for novel microorganisms able to produce LA with no side effects has been the main focus of many researches.
Considering the great demand for the development of new technologies for the purification of therapeutic enzymes, still limited by their high cost, this project aims to produce recombinant LA by bacteria fermentation and to developa cost-effective and sustainable platform for the biopharmaceutical purification (downstream processing) from the fermentation broth using nanomaterials. The main goal is to obtain recoveries of LA above 90% and purity levels over 95%, with a cost reduction around 20%. A critical evaluation of the best strategies and conditions for the integrationprocesses and scale-up will be explored.
The involved research teams (CICECO, FEUP and UNESP) have shown to be successful in providing pioneering scientific results on the production of enzymes and on the development of separation and purification techniques for biopharmaceuticals. The UNESP team from Brazil will optimise noveltechniques of heterologous enzyme production by bacterial fermentation, which is generally regarded as safe (GRAS) and easy to manipulate, in order to achieve higher enzyme yields. The Portuguese teams (CICECO and FEUP) will be responsible for the preparation of functionalisation of silica and carbon nanomaterials, which will be used as an efficient and fast strategy for LA purification from the fermentation medium. NanoPurAsp aims to meet the approval of biopharmaceuticals by regulatory authorities which require effective and high quality biopharmaceuticals, i.e., no side-effects, high purity and biological activity. These can only be attained through in-depth knowledge of the products and of their manufacturing processes. The advanced research here produced will certainly allow the success of therapeutic enzymes production and its easy scalability up to industrial levels at a lower cost. The integrated process developed will have a great impact in economy with a long-term impact in human healthcare. This project aspires the optimization of resources and materials which will contribute to a greener and sustainable growth, which in turn will be a source of economic valorisation for the future industrial manufacturing.
Regarding the candidate:
– Candidate must have PhD in Biotechnology or related disciplines;
– Have completed the PhD no longer than 2 years prior to application;
– Proven international experience in groups outside the institution of origin of the doctorate;
– Excellent early achievement track record (scientific publications and /or patent deposits).
– Experience with Molecular Biology techniques;
– Experience with cultivation and handling of microorganisms, mainly bacteria;
– Experience with submerged cultivation;
– Desirable knowledge of bioreactors.
Applications should send the documents listed below to Prof. Dr. Valéria de Carvalho Santos Ebinuma (Principal investigator) by email: [email protected] . Deadline: 12/01/2018.
1) CV lattes updated (Brazilian) or CV with academic experience (foreigners);
2) Cover letter explaining what are the applicant’s motivations to undertake the project, as well as her/his qualification;
3) References (2 contacts).
This opportunity is open to candidates of any nationalities. The selected candidate will receive a FAPESP’s Post-Doctoral fellowship in the amount of R$ 7,373.10 monthly and a research contingency fund, equivalent to 15% of the annual value of the fellowship which should be spent in items directly related to the research activity.
More information about the fellowship is at: fapesp.br/oportunidades/2480 .
Master’s Fellowship in Bioinformatics
The Laboratory of Molecular Biology from the Blood Center of Ribeirão Preto, Brazil, offers one position for a master degree student with a basic knowledge of bioinformatics. The student will be responsible for the optimization and the implementation of a pipeline for the analysis of the viral metagenome (filtering sequences, alignments, taxonomic designation, de novo genome assembly) obtained from plasma and hemoderivatives. To start the working of this project, the student will receive training by international researchers from the Pasteur Institute. The student will be supervised by Dr. Svetoslav Slavov (https://bv.fapesp.br/en/pesquisador/83362/svetoslav-nanev-slavov/ ). The scholarship and the technical reserve values are pre-establshed by the Sao Paulo Research Foundation — FAPESP.
The blood center of Ribeirão Preto is a leading research institution which is funded by FAPESP, Brazil’s National Council for Scientific and Technological Development — CNPq and the Brazilian Innovation Agency — FINEP, and has an excellent infrastructure. More than 100 researchers work in the Blood Center of Ribeirão Preto in different research areas. One of the main lines of investigations includes the infectious diseases which can be transmitted via blood transfusion and the measures which can be undertaken to prevent such a process.
Basic requirements: Linux; Program languages perl, python, and/or R; data banks; Analysis of next generation sequencing raw data is a plus.
Those who are interested in this position, please send your CV, motivation letter and one recomendation letter of the Secretariat of Research of the Blood Center of Ribeirão Preto until 20/12/2018 ([email protected] r) mentioning the running head Mestrado_FAPESP_Slav and with a copy to [email protected] .
More information about the fellowship is at: fapesp.br/oportunidades/2475 .
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